Composite

Part:BBa_Y00100:Design

Designed by: Natalie Kuldell   Group: Yeast-1   (2006-08-04)

nuclear 5-aminolevulinate synthase gene from S. cerevisiae, recoded for mitochondrial expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 694
    Illegal BglII site found at 1169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon optimized for mitochondrial gene expression, HA-epitope C term (not N-term where mt import sequences rest), flank coding seq with homology to COX3 gene of mitochondria to allow for integration and disruption of this gene (should make respiration+ cells become respiration-), and finally added BB prefix and suffix to allow cloning into std Registry vectors

  • RS 18:43, 10 September 2008 (UTC): This part's sequence was initially entered including the BioBrick prefix and suffix. The part's sequence and features have been amended to eliminate the BioBrick prefix and suffix per Registry convention.

Source

direct synthesis from DNA 2.0, summer 2006

References